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Anti-MOP3 Antibody

品牌
Sigma-Aldrich
货号
AB4140
规格纯度
Chemicon®, from rabbit
价格
6190.47 *本价格含增值税费
促销
服务
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  • 包邮
  • 增值税票
数量
-+
半岛bd体育手机客户端 名称:
Anti-MOP3 Antibody
BMAL1
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

特异性

Recognizes PAS orphan MOP3. The calculated molecular weight is ~68.8 kDa.

免疫原

Synthetic peptide corresponding to amino acids 257-272 of human MOP3. (SVKVEDKDFPSTCSKK).

应用

Research Category
Neuroscience
Anti-MOP3 Antibody detects level of MOP3 & has been published & validated for use in WB, IC, IH.
Research Sub Category
Circadian Rhythm & Sleep
Western Blot, Immunohistochemistry, Immunocytochemistry.

Recommended dilution 1/200 to 1/1000.

Optimal dilutions must be determined by the user.

外形

Affinity purified immunoglobulin precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

储存及稳定性

Maintain unopened vial at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss32160702
NACRESNA.41

半岛bd体育手机客户端 性质

生物来源rabbit
质量水平100
抗体形式affinity purified immunoglobulin
antibody product typeprimary antibodies
克隆polyclonal
纯化方式affinity chromatography
species reactivityhuman, mouse, rat
manufacturer/tradenameChemicon®
technique(s)immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
NCBI登记号NM_018183.2
UniProt登记号O00327
运输dry ice
Gene Informationhuman ... SBNO1(55206)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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