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Anti-mono- and poly-ADP-ribose binding reagent

品牌
Sigma-Aldrich
货号
MABE1075
规格纯度
fromEscherichia coli
价格
5854.74 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-mono- and poly-ADP-ribose binding reagent
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Anti-mono and poly ADP-ribose binding reagent is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). It is useful for the affinity detection of both poly-and mono-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis. The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.

特异性

This reagent binds to both mono- and poly-ADP ribosylated proteins.

应用

Anti-mono- and poly-ADP-ribose binding reagent, Cat. No. MABE1075, is a reagent that targets mono- and poly-ADP-Ribosylated proteins and has been tested in Dot Blots and Western Blotting.
Western Blotting Analysis: 0.4 ?g/mL of a representative lot detected mono- and poly-ADP ribosylated proteins.

Dot Blot Analysis: A representative lot detected mono- and poly-ADP-ribosylated proteins.

Dot Blot Analysis: A representative lot detected mono- and poly-ADP-ribosylated proteins (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).

Western Blotting Analysis: A representative lot detected mono-and poly-ribosylated proteins. (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).






Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Gel Electrophoresis.

Gel Electrophoresis Analysis: 0.5 ?g of this binding reagent was analyzed on GEL Electrophoresis to test for purity.

目标描述

Variable depending on the target proteins and the extent of ADP-ribosylation.

外形

Purified E. coli recombinant monoclonal binding reagent in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10 mM Imidazole, 1 mM PMSF, 1 mM -Mercaptoethanol without preservatives with 10% glycerol.

Format: Purified
Immobilized Metal Affinity Chromatography (IMAC)

储存及稳定性

Stable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

生物来源 Escherichia coli
质量水平 100
抗体形式 purified antibody
antibody product type primary antibodies
克隆 monoclonal
species reactivity human, mouse
包装 antibody small pack of 25 μg
technique(s) dot blot: suitable
western blot: suitable
运输 ambient

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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