Anti-mono- ADP-ribose binding reagent

半岛bd体育手机客户端 说明

一般描述

Anti-mono-ADP-ribose binding reagent is a His-tagged recombinant protein fused to rabbit Fc tag, expressed in and purified from Rosetta (DE3)pLysS strain of E. coli (Cat. No. 70956). It is useful for the affinity detection of both mono-ADP-ribosylated proteins on membranes in a manner similar to antibody-based Western and dot blot analysis. The rabbit Fc tag allows visualization of the binding with conjugated anti-rabbit secondary antibodies. The Fc tag also allows Anti-ADP-ribose binding reagent to be captured on Protein A resins for affinity pull-down applications.

特异性

This reagent binds to mono-ADP ribosylated proteins.

应用

Western Blotting Analysis: 0.4 ?g/mL of a representative lot detected mono-ADP-ribosylated proteins.

Dot Blot Analysis: A representative lot detected mono-,-ADP-ribose modified proteins.

Dot Blot Analysis: A representative lot detected mono-ADP ribosylated proteins. (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).

Western Blotting Analysis: A representative lot detected mono-ADP-ribose modified protein by Wester blotting (Courtesy of Lee Kraus, University of Texas Southwestern Medical Center).
Research Category
Epigenetics & Nuclear Function
Anti-mono-ADP-ribose binding reagent, Cat. No. MABE1076, is a reagent that targets mono (ADP-ribose) modified proteins and has been tested in Dot Blot and Western Blotting.

质量

Evaluated by Gel Electrophoresis.


Gel Electrophoresis Analysis: 0.5 ?g of this binding reagent was analyzed on GEL Electrophoresis to test for purity.

目标描述

Variable depending on the target proteins and the extent of ADP-ribosylation.

外形

Format: Purified
Immobilized Metal Affinity Chromatography (IMAC)
Purified from E. coli and supplied in buffer containing 10 mM Tris pH 7.5, 0.2 M NaCl, 10 mM Imidazole, 1 mM PMSF, 1 mM beta-Mercaptoethanol, with 10% glycerol without preservatives.

储存及稳定性

Stable for 1 year at -80°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -80°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

生物来源	Escherichia coli
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	monoclonal
species reactivity	mouse, human
包装	antibody small pack of 25 μg
technique(s)	dot blot: suitable western blot: suitable
运输	ambient

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable