Anti-MMP-9 Antibody, NT

半岛bd体育手机客户端 说明

一般描述

All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn2+ binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases).

MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes.

特异性

The antibody recognizes human MMP-9. It does not cross react with MMP-1 or MMP-2.

免疫原

KLH-conjugated synthetic peptide corresponding to the N-terminus of MMP-9.
Epitope: N-terminus

应用

Research Category
Cell Structure
Anti-MMP-9 Antibody, N-terminus is an antibody against MMP-9 for use in WB.
Research Sub Category
MMPs & TIMPs

质量

Evaluated on a representative lot by Western blot using an HL-60 cell lysate supplemented with a PMA conditioned media.

Western Blot Analysis: 0.5 ?g/ml of this antibody detected MMP-9 on 10 ?g of HL-60 plus PMA conditioned media.

目标描述

92kDa (pro-form) and 88kDa (active form)

联系

Replaces: AB805

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Antigen Affinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HL-60 plus PMA conditioned media

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	canine, human
species reactivity (predicted by homology)	rat (based on 100% sequence homology), primate (based on 100% sequence homology), monkey (based on 100% sequence homology), dog (based on 100% sequence homology), horse (based on 100% sequence homology), pig (based on 100% sequence homology)
technique(s)	western blot: suitable
NCBI登记号	NP_004985.2
UniProt登记号	P14780
运输	wet ice
Gene Information	human ... MMP9(4318)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable