Anti-MAN1B1 Antibody, clone 1D6

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一般描述

Endoplasmic reticulum (ER) mannosyl-oligosaccharide 1,2-alpha-mannosidase (EC 3.2.1.113; UniProt Q9UKM7), also known as ER mannosyl-oligosaccharide 1,2-alpha-mannosidase, ER alpha-1,2-mannosidase, ERMan1, ERManI, ER mannosidase 1, Man9GlcNAc2-specific-processing alpha-mannosidase, Mannosidase alpha class 1B member 1) is encoded by the MAN1B1 gene in human. Originally thought to function in a similar fashion as its yeast ortholog Mns1p, which removes a terminal mannose unit to initiate a glycan-based ER-associated degradation (ERAD) signal. Mammalian MAN1B1 protein is actually localized in Golgi instead of ER. MAN1B1 protein participates in a Golgi-based quality control network that captures misfolded protein substrate, which escape from ER, most likely via COPII vesicles, and recycles them back to the ER for ERAD. Upon entering the Golgi, the initially escasped unfold proteins are retained by a cargo-capturing complex in which non-enzymatic regions of MAN1B1, including the decapeptide sequence (DPS) within the MAN1B1 luminal stem, play an integral role. Facilitated by direct interactions with gamma-COP, MAN1B1 promotes the recruitment of unfold proteins into COPI vesicles, which then recycles the unfold proteins back to the ER via retrograde COPI vesicles without the need for trimming by MAN1B1 protein.

免疫原

GST-tagged recombinant protein corresponding to human MAN1B1.

应用

Western Blotting Analysis: 2.0 ?g/mL from a representative lot detected MAN1B1 in 10 ?g of PC12 cell lysate.
Immunoprecipitation Analysis: A representative lot immunoprecipitated MAN1B1 in HeLa cell lysate ((Pan, S., et al. (2011). Mol. Bio. Cell. 22(16):2810-2822).
Immunoprecipitation Analysis: A representative lot immunoprecipitated MAN1B1 in transfected HeLa cells (Iannotti, M.J., et al. (2014). J. Biol. Chem. 289(17):11844-11858).
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected MAN1B1 in HeLa cells (Pan, S., et al. (2011). Mol. Bio. Cell. 22(16):2810-2822. & Iannotti, M.J., et al. (2014). J. Biol. Chem. 289(17):11844-11858).
Research Category
Signaling
Research Sub Category
Signaling Neuroscience
Anti-MAN1B1 Antibody, clone 1D6 is an antibody against MAN1B1 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2.0 ?g/mL of this antibody detected MAN1B1 in 10 ?g of HeLa cell lysate.

目标描述

~80/90 kDa observed. The calculated molecular weight of this protein is 80 kDa, but can be observed at ~90 kDa due to post translational modifications (Pan, S., et al. (2011). Mol. Bio. Cell. 22(16):2810-2822).

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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