半岛bd体育手机客户端 说明
一般描述
The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor (Cheresh, 1991). The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented.
The beta5 subunit associates with alphaV forming a receptor for vitronectin. This complex is expressed in a wide range of cell types including fibroblast and keratinocytes. It is also expressed at high levels in several tumors including lung, pancreatic and mammary carcinomas. It is not expressed in lymphblastoid cells.
特异性
Recognizes Integrin beta5. Specificity determined by immunoprecipitation from detergent extract of 35S methionine labeled cells. No cross-reactivity to beta1 or beta3.
免疫原
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human integrin beta 5 subunit (KTFNKFNKSYNGTVD). Note : a lysine (K) residue was added to the N-terminal of the peptide to facilitate coupling to the carrier protein.
Epitope: C-terminus/cytosolic
应用
Detect Integrin β5 using this Anti-Integrin β5 Antibody, C-terminus/cytosolic validated for use in ELISA, IF, IH(P), IP, RIA & WB.
ELISA/RIA
Immunoprecipitation: 5μL of antibody is sufficient to precipitate alphaVbeta5 from
2.5x10E6 cells.
Western blot: non-reduced conditions; do not use milk in the dilution buffer.
Immunofluorescence: 1:100.
Immunohistochemistry (Acetone Fixed) 1:40-1:80
Immunohistochemistry (Paraffin Embedded) 1:200 (ABC)
Optimal working dilutions must be determined by end user.
目标描述
88 kDa
外形
UnPurified whole rabbit Serum containing 0.05% sodium azide as a preservative.
Unpurified
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | serum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | rat, mouse, hamster, human |
| manufacturer/tradename | Chemicon? |
| technique(s) | ELISA: suitable immunofluorescence: suitable immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable radioimmunoassay: suitable western blot: suitable |
| NCBI登记号 | NM_002213.3 |
| UniProt登记号 | P18084 |
| 运输 | dry ice |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
Sigma-Aldrich
m.cnreagent.com
