Anti-Importin alpha-3/Qip1 Antibody, clone 3D10

半岛bd体育手机客户端 说明

一般描述

Importin subunit alpha-3 (UniProt O35343; also known as Importin alpha Q1, Karyopherin subunit alpha-4, Qip1) is encoded by the Kpna4 (also known as Qip1) gene (Gene ID 16649) in murine species. The transport of macromolecules between cytoplasm and nucleus is mediated by importins and exportins and regulated by the small GTPase Ran. Importins bind their cargoes with either nuclear localization signal (NLS) or M9 domain-containing hnRNP proteins in the cytoplasm, while exportins target their substrates via their nuclear export signal (NES) or M9 domain inside the nucleus. Qip1/KPNA4 belongs to the importin- /karyopherin- (KPNA) family of adaptor proteins that interact with both monopartite and bipartite types of classical nuclear localization signals (cNLSs). The seven KPNA family members are divided into the 1 (KPNA1/5/6), 2 (KPNA2/7), and 3 (KPNA3/4) subfamilies based on structural and functional similarities. KPNA members mediate substrate cargo nuclear import by bridging substrate cargo and the Ran-karyopherin- complex. Both KPNA3 and Qip1/KPNA4 are reported to target methyl-CpG-binding protein 2 (MeCp2) bipartite NLS (255-RKAEADPQAIPKKRGRK-271). Qip1/KPNA4 can ineract with both wild-type and MeCp2 R270X mutants found in Rett syndrome (RTT) patients, while KPNA3 binds only wild-type, but not MeCp2 R270X mutants. Human and murine Qip1 contains an NLS (a.a. 43-52) in its importin- -binding (IBB) domain (a.a. 2-58), followed by ten ARM domains (a.a. 66-485), one majore and one minor NLS-binding site (a.a. 137-229 and a.a. 306-394).

特异性

Clone 3D10 detected recombinant mouse importin 3/Qip1, but not importin 1/Rch1, importin 5/NPI-1, or importin 7/S2 GST fusion proteins (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400).

免疫原

Recombinant full-length mouse Importin alpha-3/Qip1.

应用

Detect Importin subunit alpha-3/Qip1 using this rat monoclonal Anti-Importin alpha-3/Qip1, clone 3D10, Cat. No. MABS1326, validated for use in Western Blotting.
Western Blotting Analysis: A representative lot detected recombinant mouse importin 3/Qip1, but not importin 1/Rch1, importin 5/NPI-1, or importin 7/S2 GST fusion proteins (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400).

Western Blotting Analysis: A representative lot detected endogenous importin 3/Qip1 in human HeLa, monkey COS-7, mouse L-929, rat NRK , hamster BHK, and bovine MDBK cell lysates (Sakaguchi, N., et al. (2003). Hybrid. Hybridomics. 22(6):397-400).

Research Category
Signaling

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected in 10 ?g of HeLa cell lysate.

目标描述

~58 kDa observed. 57.76 kDa (human) and 57.79 kDa (mouse & rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified.
Purified rat IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

质量水平	100
生物来源	rat
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	3D10, monoclonal
species reactivity	hamster, human, rat, mouse, bovine, monkey
species reactivity (predicted by homology)	canine (based on 100% sequence homology)
technique(s)	western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_032493
UniProt登记号	O35343
运输	ambient

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable