半岛bd体育手机客户端 说明
一般描述
Mouse monoclonal antibody purified by ion-exchange chromatography. Recognizes the ~70 kDa Hsp70 protein.
Anti-Hsp70, mouse monoclonal, clone C92F3A-5, recognizes the ~70 kDa Hsp70. Does not cross-react with Hsc70. It is validated for use in ELISA, FC, WB, ICC, IP & IHC (frozen and paraffin sections).
Recognizes the ~70 kDa Hsp70 protein. Does not cross-react with Hsc70.
免疫原
Hsp70 from HeLa cells
Human
包装
50 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
ELISA (see comments)
Flow Cytometry (see comments)
Frozen Sections (5 ?g/ml)
Immunoblotting (1 ?g/ml)
Immunocytochemistry (5 ?g/ml)
Immunoprecipitation (see comments)
Paraffin Sections (5 ?g/ml)
警告
Toxicity: Standard Handling (A)
外形
In PBS, 50% glycerol, pH 7.2.
重悬
Following iniital thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
L929 cells, Human colon cancer tissue
其他说明
Does not cross-react with Hsc70. Because Hsp70 is not expressed constitutively in most cells, this antibody is suitable for ascertaining whether a stress-response has occurred in the cell. Increased levels of Hsp70 expression occurs following stress even in cells that constitutively express Hsp70. This antibody has also been reported to work for ELISA, flow cytometry, and immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
Hang, H., and Fox, M.H. 1995.Cytometry19,119.
Kilgore, J.L., et al. 1994.J. Appl. Physiol.76,589.
Heufelder, A.E., et al. 1992.J. Clin. Endocrinol. Metab.74,724.
Gower, D.J., et al. 1989.J. Neurosurg.70,605.
Milarski, K., et al. 1989.J. Cell Biol.108,413.
Vass, K., et al. 1988.Acta Neuropathologica77,128.
Welch, W.J., and Suhan, J.P. 1986.J. Cell Biol.103,2035.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | C92F3A-5, monoclonal |
| 形式 | liquid |
| 包含 | ≤0.1% sodium azide as preservative |
| species reactivity (predicted by homology) | all |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
| 同位素/亚型 | IgG1 |
| 运输 | wet ice |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
