半岛bd体育手机客户端 说明
一般描述
Phospholipid hydroperoxide glutathione peroxidase, cytosolic (UniProt: P36969-2; also known as PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4) is encoded by the GPX4 gene (Gene ID: 2879) in human. Three functionally distinct isozymes of GPx4 have been described and are known as mGPx4 (mitochondrial), cGPx4 (cytosolic), and nGPx4 (nuclear). They originate from a single GPX4 gene.
The cytosolic isoform is ubiquitously expressed at moderate levels in most mammalian cells. On the other hand, mitochondrial and nuclear isoforms are overexpressed in spermatoid cells. Clone Mab63-1 specifically recognizes cGPx4 in human and its ortholog in mouse. However, it does not recognize rat GPx4. GPx4 is a considered to be a moonlighting protein that has been implicated in anti-oxidative defense, gene expression regulation, and programmed cell death, and as a structural protein in spermatogenesis. Homozygous GPx4-deficient mice are reported to die utero before E7.5. Similarly, homozygous mice expressing catalytically inactive GPx4 variants (Sec46Ala/Ser mutant) do not survive beyond the 7th day of embryogenesis. (Ref.: Borchert, A., et al (2010). Immunol. Lett. 133 (2); 85-93).
特异性
Clone Mab63-1 specifically detects cytosolic Glutathione Peroxidase 4 in human and murine cells. it targets a minimum epitope of 6 amino acids from the N-terminal region.
免疫原
His-tagged purified recombinant human cytosolic glutathione peroxidase 4.
应用
Anti-Glutathione Peroxidase 4, clone Mab63-1, Cat. No. MABF1969, is a mouse monoclonal antibody that detects cytosolic glutathione peroxidase 4 and has been tested for use in Dot Blot, Electron Microscopy, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Glutathione Peroxidase 4 in human testis and mouse testis tissues.
Immunoprecipitation Analysis: A representative lot detected Glutathione Peroxidase 4 in Immunoprecipitation applications (Borchert, A., et. al. (2010). Immunol Lett. 133(2):85-93).
Immunohistochemistry Analysis: A representative lot detected Glutathione Peroxidase 4 in Immunohistochemistry applications (Borchert, A., et. al. (2010). Immunol Lett. 133(2):85-93; Brutsch, S.H., et. al. (2015). Antioxid Redox Signal. 22(4):281-93).
Western Blotting Analysis: A representative lot detected Glutathione Peroxidase 4 in Western Blotting applications (Brutsch, S.H., et. al. (2015). Antioxid Redox Signal. 22(4):281-93; Borchert, A., et. al. (2010). Immunol Lett. 133(2):85-93).
Dot Blot Analysis: A representative lot detected Glutathione Peroxidase 4 in Dot Blot applications (Borchert, A., et. al. (2010). Immunol Lett. 133(2):85-93).
Electron Microscopy Analysis: A representative lot detected Glutathione Peroxidase 4 in Electron Microscopy applications (Brutsch, S.H., et. al. (2016). J Biol Chem. 291(45):23578-23588).
Research Category
Inflammation & Immunology
质量
Evaluated by Western Blotting in HEK293 cells transfected with Gpx4.
Western Blotting Analysis: 2 ?g/mL of this antibody detected Glutathione Peroxidase 4 in lysate of HEK293 cells transfected with Gpx4.
目标描述
~19 kDa observed; 19.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外形
Protein G purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | Mab63-1, monoclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | mouse, human |
| 包装 | antibody small pack of 25 μg |
| technique(s) | dot blot: suitable electron microscopy: suitable immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable |
| 同位素/亚型 | IgG2aκ |
| NCBI登记号 | NP_002076.2 |
| UniProt登记号 | P36969-2 |
| 运输 | ambient |
Sigma-Aldrich
m.cnreagent.com
