Anti-GluR2 Antibody, clone L21/32

半岛bd体育手机客户端 说明

一般描述

Glutamate receptors (GluRs) can be categorized as ionotropic or metabotropic and subcatergorized by their agonist preferences (NMDA, AMPA or Kainic acid). There are four types of AMPA selective GluR subunits (GluR1, GluR2, GluR3 and GluR4). Tetrameric or pentameric combinations of different subunits contributes to the functional diversity of AMPA receptors. In general, AMPA receptors mediate fast synaptic current at most excitatory synapses, with stoichiometry characterized by subtype composition. Although subunit composition of AMPA receptors varies, they must contain at least one edited GluR2 subunit to be calcium impermeable. The critical residue controlling calcium permeability is in the pore loop region. In GluR1, GluR3, and GluR4, this position is occupied by a Gln residue. In GluR2, it is occupied by an Arg residue. It has been shown experimentally that the presence of Arg in this position blocks Ca2+ ion permeability, while a Gln does not. Relative calcium permeability in AMPA receptor channels may be significant in pathological neurotoxic damage and long term changes in nervous system responses.

免疫原

Recombinant protein corresponding to human GluR2.

应用

Research Sub Category
Neurodegenerative Diseases

Neurotransmitters & Receptors
Research Category
Neuroscience

Neuroscience
Detect GluR2 using this Anti-GluR2 Antibody, clone L21/32 validated for use in WB, IH.
Immunohistochemistry Analysis: 1:300 dilution of this antibody from a previous lot detected GluR2 in rat cerebellum and hippocampus tissue.

质量

Evaluated by Western Blot in rat brain membrane tissue lysate.

Western Blot Analysis: 0.5 ?g/mL of this antibody detected GluR2 on 10 ?g of rat brain membrane tissue lysate.

目标描述

~ 96 kDa observed

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Rat brain membrane tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	L21/32, monoclonal
species reactivity	rat
species reactivity (predicted by homology)	human (immunogen homology), mouse (based on 100% sequence homology)
technique(s)	immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_000817
UniProt登记号	P42262
运输	wet ice
Gene Information	human ... GRIA2(2891)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable