半岛bd体育手机客户端 说明
一般描述
Anti-Giα-3-Subunit, C-Terminal (345-354), rabbit polyclonal, recognizes Giα-3 subunit. Does not cross-react with Gsα & Goα subunits. Validated for use in WB & IP.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the Giα-3 subunit protein.
Recognizes Giα-3 subunit. Does not cross-react with Gsα and Goα, but partially cross-reacts with Giα1-, G1α2-subunits.
免疫原
a synthetic peptide (CKNNLKECGLY) corresponding to amino acids at the C-terminus of mammalian Giα-3, conjugated to KLH
包装
50 μL in Glass bottle
应用
Immunoblotting (1:1000)
Immunoprecipitation (see comments)
警告
Toxicity: Standard Handling (A)
外形
In 140 mM NaCl, 100 mM potassium phosphate, pH 7.5.
重悬
Following initial thaw, aliquot and freeze (-20°C).
其他说明
Kumar, R., et al. 1994.J. Mol. Cell. Cardiol.26,1537.
Raymond, J.R., et al. 1993.Biochemistry32,11064.
Mumby, S.M., and Gilman, A.G. 1991.Methods Enzymol.195,215.
Jones, D.T., and Reed, R.R. 1987.J. Biol. Chem.262,14,241.
The specificity was confirmed with lysates from separate cultures of bacteria transfected with the genes for Gsα, Giα-1, GIα-2, Giα-3, and Goα. The original titer of the serum was 1:10,000. Suitable for immunoblotting and immunoprecipitation. Does not cross-react with Gsα and Goα. Antibody partially cross-reacts with Giα-1 and GIα-2. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | affinity isolated antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
形式 | liquid |
不包含 | preservative |
species reactivity (predicted by homology) | mammals |
manufacturer/tradename | Calbiochem? |
储存条件 | OK to freeze avoid repeated freeze/thaw cycles |
同位素/亚型 | IgG |
运输 | wet ice |
储存温度 | ?70℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich