Anti-F-actin-capping protein subunit alpha-1 Antibody

半岛bd体育手机客户端 说明

一般描述

F-actin capping protein subunit alpha (CapZ alpha) belongs to the F-actin capping protein family, which are characteristic heterodimers, consisting of an α subunit (31-36 kDa) and a β subunit (28-32 kDa), that cap the barbed end of actin filaments within all eukaryotes. Their ability to bind the actin filaments is in a manner independent of Ca2++ and requires the C-terminal end of both subunits for optimal binding. CapZ is contained within the Z-discs of striated muscle, where it functions to inhibit the polymerization and depolymerization of actin. Within vertebrates, there are three isoforms for each of CapZ’s subunits. All three alpha subunit isoforms are components of the WASH complex. Little is known about isoforms 1 and 2; however, isoform 3 is thought to be involved in spermatid morphogenesis and is exclusively observed within sperm and testis, with highest expression within the neck region of sperm.

免疫原

GST-tagged recombinant protein corres-ponding to human F-actin-capping protein subunit alpha-1.

应用

Research Category
Cell Structure
Detect F-actin-capping protein subunit alpha-1 using this Anti-F-actin-capping protein subunit alpha-1 Antibody validated for use in WB & IC.
Research Sub Category
Cytoskeleton
Western Blot (Snap i.d. ) Analysis: 1 ?g/mL from a previous lot detected F-actin-capping protein subunit alpha-1 on 10 ?g of HeLa cell lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected F-actin-capping protein subunit alpha-1 in NIH/3T3, HeLa, and A431 cells.

质量

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.1 ?g/mL of this antibody detected F-actin-capping protein subunit alpha-1 on 10 ?g of HeLa cell lysate.

目标描述

~35 kDa

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, pig, mouse, rat
species reactivity (predicted by homology)	bovine (based on 100% sequence homology), porcine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), primate (based on 100% sequence homology), canine (based on 100% sequence homology)
technique(s)	immunocytochemistry: suitable western blot: suitable
UniProt登记号	P52907
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable