Anti-Diglycyl Lysine (Clone GX41) Antibody

半岛bd体育手机客户端 说明

一般描述

Anti-Diglycyl Lysine has been shown to specifically detect peptides derived from the ubiquitinated portion of proteins. Diglycyl Lysine, clone GX41 is thought to find diglycyl-lysine moieties that are created and left behind at places of ubiquitination after digestion with trypsin has occurred.

特异性

Antibody recognizes lysine residues modified by diglycine, a residue left at sites of ubiquitination after trypsin digestions.
Broad species cross-reactivity expected based on 100% sequence homology.

免疫原

Linear peptide corresponding to Diglycyl Lysine.

应用

Research Sub Category
General Post-translation Modification
Research Category
Signaling
Immunoprecipitation Analysis: 10 ?g of Anti-Diglycyl Lysine immunoprecipitated peptides containing Diglycyl Lysine modification from isolated ubiquitinated proteins which were obtained from a human cell line.

Mass Spectrometry Analysis: A previous lot was used by an independent a laboratory in Mass Spec. Image courtesy of Samie Jaffrey, Cornell Medical College.
Anti-Diglycyl Lysine (Clone GX41) Antibody detects level of Diglycyl Lysine (Clone GX41) & has been published & validated for use in WB & IP.

质量

Evaluated by Western Blot in BSA protein and Diglycine modified BSA.

Western Blot Analysis: 0.5 ?g/mL of this antibody detected Diglycyl Lysine on 10 ?g of BSA protein and Diglycine modified BSA.

目标描述

Molecular weight can vary depending on the size of the protein or peptides containing Diglycyl modified lysine residues.

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
BSA protein and Diglycine modified BSA

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	GX41, monoclonal
species reactivity	rat, human, mouse
species reactivity (predicted by homology)	all
technique(s)	immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG1κ
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable