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位置: 首页> 品牌> Sigma-Aldrich> Anti-Cas9 Antibody, NT Antibody, clone 8C1-F10

Anti-Cas9 Antibody, NT Antibody, clone 8C1-F10

品牌
Sigma-Aldrich
货号
MABE1669
规格纯度
clone 8C1-F10, from mouse
价格
4940.37 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-Cas9 Antibody, NT Antibody, clone 8C1-F10
CRISPR-associated endonuclease Cas9/Csn1, SpyCas9
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

CRISPR-associated endonuclease Cas9/Csn1 (UniProt Q99ZW2; also known as SpyCas9) is encoded by the Streptococcus pyogenes serotype M1 gene Cas9 (also known as csn1) gene (Gene ID 901176). Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA loci containing short repetitions of base sequences complementary to antecedent mobile elements and target invading nucleic acids. Each repetition is followed by short segments of spacer DNA from previous exposures to a virus. Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1′s CRISPR immune system that confers resistance to foreign genetic elements such as plasmids and phages. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems, correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and Cas9. Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3′-5′ exonucleolytically.

特异性

Clone 8C1-F10 targets an epitope within Cas9 N-terminal region outside the active site RuvC-I.

免疫原

His-tagged recombinant Cas9 N-terminal fragment excluding the active site RuvC-I.

应用

Anti-Cas9, NT, clone 8C1-F10 , Cat. No. MABE1669, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9/Csn1 and has been tested in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected exogenously expressed full-length nuclease-dead Cas9 in transfected NIH/3T3 cells fixed with 4% paraformaldehyde and permeabilized by 0.3% Triton X-100.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in NIH3T3 cells.

Western Blotting Analysis: 0.25 ?g/mL of this antibody detected exogenously expressed full-length nuclease-dead Cas9 in 10 ?g of lysate from transfected NIH/3T3 cells.

目标描述

~160 kDa observed. 158.4 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein G purified.
Purified mouse IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 8C1-F10, monoclonal
species reactivity Streptococcus pyogenes
species reactivity (predicted by homology) all
technique(s) immunocytochemistry: suitable
western blot: suitable
同位素/亚型 IgG2bκ
NCBI登记号 NP_269215
UniProt登记号 Q99ZW2
运输 ambient

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

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