Anti-Brm Antibody, clone 1H7A10

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一般描述

BAF190B (BRG1-associated factor 190B), also known as protein brahma homolog (Brm), and SNF2-alpha, functions as a transcriptional coactivator. This protein contains a helicase domain and is a member of the SWI/SNF family of proteins and is part of the SWI-SNF chromatin remodeling complex. The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. This large complex contains multiple proteins. BAF190 (Brm) and Brg-1 (SNF2-beta) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, or SNF5), BAF155 (SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).

特异性

Recognizes BAF190B also known as Brm

免疫原

Recombinant protein corresponding to amino acids 48-214 of mouse Brm.

应用

Use Anti-Brm Antibody, clone 1H7A10 (Rat Monoclonal Antibody) validated in WB, IHC to detect Brm also known as ATP-dependent helicase SMARCA2 or SNF2/SWI2-like protein 2.
Immunocytochemistry:
A representative lot of this antibody was reported to work in IC using C2C12 cells (Yasuyuki Ohkawa, SSP Stem Cell Unit, Faculty of Medicine, Kyushu University, Fukuoka, Japan.)

Immunohistochemistry:
A representative lot of this antibody was reported to work in IHC using mouse embryonic and postnatal brain (Yasuyuki Ohkawa, SSP Stem Cell Unit, Faculty of Medicine, Kyushu University, Fukuoka, Japan.)

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

质量

Evaluated by Western Blot in K562 nuclear extract.

Western Blot Analysis: 0.5 ?g/mL of this antibody detected Brm on 10 ?g of K562 nuclear extract.

目标描述

~140-180 kDa observed. Uncharacterized lower molecular weight bands have been reported in some lysates and may represent splice variants and/or degradation products. See Batsché E, Yaniv M, Muchardt C. Nat Struct Mol Biol. 2006 Jan;13(1):22-29.

外形

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
K562 nuclear extract

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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