半岛bd体育手机客户端 说明
一般描述
Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: BimEL, BimL, and BimS. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. BimEL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of BimELby ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.
特异性
Rat BimELwhen phosphorylated at serine 65. Human BimELwhen phosphorylated at serine 69. Mouse BimEL(100% conserved) has not been tested, but is expected to react.
免疫原
The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat BimELthat contains serine 65 (serine 69 in the human sequence). The sequence is conserved in human and mouse.
应用
Research Category
Apoptosis & Cancer
Research Sub Category
BCL2 & Inhibition
For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.
Anti-BimEL (pS69) human / (pS65) rat Antibody is an antibody against BimEL (pS69) human / (pS65) rat for use in WB.
质量
Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a BimEL-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).
外形
Dulbecco′s phosphate buffered saline (without Mg2+and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide
储存及稳定性
Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.
分析说明
Control
Hek293T co-expressing rat BimELand active MEKK1 (MEKK1δ).
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | purified immunoglobulin |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | human, rat |
| species reactivity (predicted by homology) | mouse (100% homologous) |
| manufacturer/tradename | Chemicon? |
| technique(s) | western blot: suitable |
| NCBI登记号 | NM_006538.3 NM_138621.3 NM_138622.2 NM_138623.2 NM_138624.2 NM_138626.2 NM_138627.2 NM_207002.2 NM_207003.1 |
| UniProt登记号 | O43521 |
| 运输 | dry ice |
| Gene Information | human ... BCL2L11(10018) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
Sigma-Aldrich
m.cnreagent.com
