半岛bd体育手机客户端 说明
一般描述
Tubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division, and intracellular trafficking. There are at least three types of tubulins: alpha, beta, and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents. Class III β-tubulin? is a microtubule element expressed exclusively in neurons, and is a popular marker specific for neurons in tissue.
特异性
Recognizes Beta III Tubulin
免疫原
Epitope: (Similar to TuJ1 antigen)
Synthetic peptides from human/rat Beta III Tubulin.
应用
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Anti-Beta III Tubulin Antibody detects level of Beta III Tubulin & has been published & validated for use in IC, IH & WB.
Immunocytochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunocytochemistry using 2% paraformaldehyde fixed cells.
Immunohistochemistry:
1:1000 - 1:2000 dilution from a previous lot was used in immunohistochemistry using 2% paraformaldehyde fixed tissues.
Optimal working dilutions must be determined by the end user
质量
Routinely evaluated by Western Blot on mouse brain lysates.
Western Blot:
1:1000 dilution of this lot detected TUBULIN BETA III on 10 μg of mouse brain lysates.
目标描述
55 kDa
联系
Replaces: 04-1049
外形
ImmunoAffinity Purified
Affinity purified polyclonal chicken IgY liquid in PBS, pH 7.2 with 0.02% sodium azide.
Four different antibodies were combined to make this product
储存及稳定性
Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
It is recommended that the antibody be stored in the dark. During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 ?L or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.
分析说明
Control
Brain tissue, Neuronal culture
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
生物来源 | chicken |
质量水平 | 100 |
抗体形式 | affinity purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | polyclonal |
纯化方式 | affinity chromatography |
species reactivity | mouse, rat, human |
manufacturer/tradename | Chemicon? |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
同位素/亚型 | IgY |
NCBI登记号 | NM_006086.2 |
UniProt登记号 | Q13509 |
运输 | wet ice |
安全信息
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 2 |
Sigma-Aldrich