Anti-Bcl-2-associated transcription factor 1 Antibody

半岛bd体育手机客户端 说明

一般描述

Bcl-2-associated transcription factor 1 (Btf) is a ubiquitously expressed death-promoting transcriptional repressor. It has been shown to interact with anti-apoptotic proteins related to Bcl-2, DNA, adenovirus E1B 19K, and EMD. Btf has also been shown to be involved in E1A transformation of p53 or E1B 19K, an interaction that implicates Btf as being involved in transcriptional regulation and in promoting apoptosis. Expression of Btf is absent in some cancers, which may suggest a potential role for the protein in tumor suppression.

特异性

Other homologies: Rat (81% sequence homology).

免疫原

KLH-conjugated linear peptide corresponding to human Bcl-2-associated transcription factor 1.

应用

Western Blot (Snap i.d.) Analysis: 0.5 ?g/mL from a previous lot detected Bcl-2-associated transcription factor 1 in 10 ?g of HeLa cell lysate.

Immunoprecipitation Analysis: 10 ?g of this antibody immunoprecipitated Bcl-2-associated transcription factor 1 from HeLa cell lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected Bcl-2-associated transcription factor 1 in neuronal and glial cells in normal human brain tissue.
This Anti-Bcl-2-associated transcription factor 1 Antibody is validated for use in WB, IP, IH(P) for the detection of Bcl-2-associated transcription factor 1.
Research Sub Category
BCL2 & Inhibition
Research Category
Apoptosis & Cancer

质量

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 ?g/mL of this antibody detected Bcl-2-associated transcription factor 1 in 10 ?g of HeLa cell lysate.

目标描述

~120 kDa observed.
This protein has 4 isoforms: Isoform 1 (106 kDa calculated), Isoform 2 (106 kda calculated), Isoform 3 (100 kDa calculated), Isoform 4 (86 kDa calculated).

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human, mouse
species reactivity (predicted by homology)	bovine (based on 100% sequence homology), canine (based on 100% sequence homology)
technique(s)	immunohistochemistry: suitable (paraffin) immunoprecipitation (IP): suitable western blot: suitable
NCBI登记号	NP_055554
UniProt登记号	Q9NYF8
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable