Anti-Atg13 Antibody, clone 2H4.2

半岛bd体育手机客户端 说明

一般描述

The autophagy-related protein 13 (Atg13) plays an important role in the formation of autophagosomes. Autophagosomes are formed in response to nutrient deprivation and function as the transport vesicles for organelles, proteins, and protein complexes targeted for lysosomes that digest these cargos to produce energy and nutrients. Atg13 is activated by the mTOR pathway and forms a complex with the FIP200 protein. This complex is involved in enhancing the activity of the ULK1 kinase which is required for the formation of autophagosomes. Atg13/FIP200 faciliates the localization of ULK1 to pre-autophagosomes, and subsequently stabilizes ULK1. Autophagy is an important process in development, growth, and cell differentiation, and disruption of this process may contribute to cancer, aging, and neurodegenerative diseases.

免疫原

GST-tagged recombinant protein corresponding to human Atg13.

应用

Western Blot Analysis: A representative lot detected Atg13 in HeLa cell lysate.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Atg13 in normal human substancia nigra tissues.
Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
This Anti-Atg13 Antibody, clone 2H4.2 is validated for use in Western Blotting, IHC for the detection of Atg13.

质量

Evaluated by Western Blot in campthothecin treated HeLa cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Atg13 in 10 ?g of campthothecin treated HeLa cell lysate.

目标描述

~60 kDa observed

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Campthothecin treated HeLa cell lysate.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	2H4.2, monoclonal
species reactivity	human
technique(s)	immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_001136145
UniProt登记号	O75143
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable