半岛bd体育手机客户端 说明
特异性
Recognizes Amyloid Precursor Protein (APP), C-terminal. Recognizes full length APP and C-terminal fragments resulting from cleavage by secretase. May react with APLP1 and APLP2.
免疫原
Epitope: C-terminus
Nine amino acid peptide from the C-terminus of APP (YKFFEQMQN)
应用
Detect Amyloid Precursor Protein using this Anti-Amyloid Precursor Protein Antibody, C-terminus validated for use in IC, IH, IP & WB.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Immunohistochemistry: 1:100-1:400
Immunocytochemistry on NTera2 and COS cell lines: 1:100-1:400
Western blot: 1:500-1:2000: The low abundance of full length APP in untreated cells means that poor response is seen with immunoblotting of whole cell lysates. We recommend that membrane fractions be prepared to increase the loading of APP as well as remove potential interferences from soluable proteins. NTera2, Cos7 and Hela cell membrane preparations are positive by western blots.
Immunoprecipitation: 1:100-1:400.
Optimal working dilutions must be determined by end user.
外形
Unpurified
Serum. Liquid in PBS with 0.02% azide.
储存及稳定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Brain
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | serum |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| species reactivity | human, monkey |
| manufacturer/tradename | Chemicon? |
| technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
| NCBI登记号 | NM_000484.2 NM_201413.1 NM_201414.1 |
| UniProt登记号 | P05067 |
| 运输 | dry ice |
| Gene Information | human ... APP(351) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
