Anti-Aldolase C Antibody, C-Term, clone 1A1

半岛bd体育手机客户端 说明

一般描述

Fructose-bisphosphate aldolase C (EC 4.1.2.13; UniProt P09972; also known as Aldolase 3, Brain-type aldolasefructoaldolase C, Fructose-1,6-biphosphate triosephosphate lyase) is encoded by the ALDOC (also known as ALDC) gene (Gene ID 230) in human. Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate. Three aldolase genes (ALDOA, ALDOB, ALDOC) are found in mammals that encode aldolase A, B, and C. Aldolase A is generally considered to be a muscle enzyme, aldolase B a liver enzyme, while aldolase C shows tissue-specific expression in adult brain with low activity in fetal tissues. Aldolase C expression is found in gray matter astrocytes and cells of the pia mater. Aldolase A and C mRNA expressions are mutually exclusive in murine central nervous system by in situ hybridization analysis, with aldolase A mRNA being detected in neurons and aldolase C in astrocytes, Purkinje cells, and some cells of the pia mater. Aldolase C can therefore be a useful marker of astrocytes, although Purkinje cells of the cerebellum also contain high levels of aldolase C.

免疫原

Epitope: Near C-terminus; 23 amino acids of aldolase C protein
Recombinant protein corresponding to human Aldolase C near the C-terminus.

应用

This Anti-Aldolase C Antibody, C-Term, clone 1A1 is validated for use in Western Blotting, Immunocytochemistry for the detection of Aldolase C.
Western Blotting Analysis: 0.5 ?g/mL from a representative lot detected Aldolase C in 10 ?g of human brain tissue lysate.
Immunocytochemistry: A representative lot selectively stained Aldolase C-positive astrocytes in mixed neuron/glial cultures.

质量

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected Aldolase C in 10 ?g of rat brain tissue lysate.

目标描述

~39 kDa observed

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing PBS with 0.05% sodium azide with 50% glycerol.
Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	1A1, monoclonal
species reactivity	human, rat
technique(s)	immunocytochemistry: suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_005156
UniProt登记号	P09972
运输	dry ice
Gene Information	human ... ALDOC(230)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable