Anti-AIF Antibody, NT

半岛bd体育手机客户端 说明

特异性

Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (Zamzami & Kroemer 1999). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (Susin et al. 1999). Like the critical molecules, cytochrome c and caspase 9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins, cytochrome c and caspase-9. AIF induces chromatin condensation and DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection. AIF is highly conserved between human and mouse and is widely expressed (Susin et al. 1999).

免疫原

Rabbit anti-AIF (N-terminus of mature AIF) polyclonal antibody was raised against a peptide corresponding to amino acids 109 to 122 of human AIF (Susin et al. 1999).
Epitope: N-terminus

应用

Research Sub Category
Apoptosis - Additional
This Anti-AIF Antibody, N-terminus is validated for use in WB for the detection of AIF.
Western blot: 0.25 to 1 μg/mL.

K562 cell lysate from HeLa cells can be used as a positive control and a 67 kDa band should be detected.

Optimal working dilutions must be determined by end user.
Research Category
Apoptosis & Cancer

联系

Replaces: 04-430

外形

Format: Purified
Purified IgG by ion exchange chromatography. Liquid in PBS containing 0.02% sodium azide.

储存及稳定性

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	western blot: suitable
NCBI登记号	NM_004208.2 NM_145812.1 NM_145813.1
UniProt登记号	O95831
运输	wet ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable