半岛bd体育手机客户端 说明
一般描述
Actin is a 43kDa highly conserved structural protein. There are three main actin isotypes (alpha,beta and gamma) which show >90% amino-acid (aa) homology between isotypes and >98% homology within members of a particular isotypic group. The majority of the isotype heterogeneity is located in the amino-terminal 30 aa. The different actin isotypes have been shown to behave very differently in vitro and in vivo. Recent studies describe differential subcellular localization of gamma-actin and isotype specific binding of actin associated proteins. {Pardo et al, 1983, Cell 32:1093-1103 & Otey, CA et al 1986 J Cell Biol 102:1726-1737, Bassell et al, 1998, J Neurosci 18:251-265 {available online}. Furthermore, there are at least six actin isoforms in vertebrates: cardiac alpha-actin and skeletal-alpha actin (expressed in striated muscle), vascular alpha-actin and enteric gamma-actin (expressed in smooth muscle), and cytoplasmic beta- and gamma-actins.{http://www.cbil.upenn.edu/MTIR/gammaActin_E-toc.html}
特异性
Recognizes actin, gamma. Specificity for gamma actin determined by 2D gel electrophoresis.
免疫原
Epitope: gamma
Synthetic peptide corresponding to the amino terminus of gamma actin {EEEIAALVIDNGSGGSG} conjugated to KLH.
应用
Research Category
Cell Structure
Western Blot: 1:500-1:2,000 or greater; non-specific bands can occur when primary antibody is overloaded. Blocking with purified milk proteins preferred over commerical concentrated milk solutions. Detects a 42-43kDa band. Specificity for gamma actin determined by 2D gel electrophoresis.
Immunocytochemistry: 1:500-1:2,000. Cultured cells fixed with PFA, then permeabilized with methanol.
Immunohistochemistry: 1:500-1:2,000. Tissues frozen and then fixed with PFA followed by Triton permeabilisation. 10% buffered formalin fixed tissues have also been used.
Optimal working dilutions must be determined by the end user.
Anti-Actin Antibody, γ is an antibody against Actin for use in IC, IH & WB.
Research Sub Category
Cytoskeleton
目标描述
42-43kDa
外形
Unpurified
Serum. Liquid, containing no preservatives.
储存及稳定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析说明
Control
Smooth muscle
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | sheep |
抗体形式 | serum |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human, mouse, rat |
manufacturer/tradename | Chemicon? |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable |
NCBI登记号 | NM_001614.2 |
UniProt登记号 | P63261 |
运输 | wet ice |
Gene Information | human ... ACTG1(71) |
安全信息
储存分类代码 | 10 - Combustible liquids |
Sigma-Aldrich