Anti-ACE2 Antibody, clone 4G5.1

半岛bd体育手机客户端 说明

一般描述

Angiotensin-converting enzyme 2 (ACE2) is a transmembrane protein belonging to the metallopeptidases (M2) family. It is a membrane-associated carboxypeptidase, expressed in the vascular endothelial cells of the heart, kidney, testis, gastrointestinal system and in the epithelial cells of lungs. ACE2 consists of an N-terminal peptidase domain (PD) and a C-terminal collectrin-like domain (CLD). It also comprises a single catalytic domain that is similar to that of ACE. The gene ACE2 is mapped to human chromosome location Xp22.2. ACE2 is a structural homolog ACE.

特异性

This antibody recognizes ACE2.

免疫原

GST-tagged recombinant protein corresponding to human ACE2.

应用

Anti-ACE2 Antibody, clone 4G5.1 detects level of ACE2 & has been validated for use in Western Blotting.
ELISA Analysis: A representative lot detected ACE2 in a direct ELISA.
Research Category
Signaling
Research Sub Category
Signaling Neuroscience

生化/生理作用

Angiotensin-converting enzyme-2 (ACE2) produces angiotensin (1-9) by hydrolysis of angiotensin 1. Angiotensin (1-9) is a precursor for angiotensin (1-7). ACE2 may also directly produce angiotensin (1-7) by conversion of angiotensin II, which is involved in vasoconstriction.(2) Angiotensin (1-7) plays an important role in the renin-angiotensin system (RAS) signaling pathway and is involved in bradykinin-induced vasodilation. Hence, ACE2 antagonizes the effects of its homolog, ACE. ACE2 is insensitive to commonly used inhibitors such as lisinopril and captopril. This gene is upregulated in heart failure due to the overactivity of angiotensin II. ACE2 is studied for its potential therapeutic value for hypertension and heart failure. It binds to the viral envelope protein spike (S) protein and acts as a major entry receptor for severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 in human cells. Hence, it is considered as a potential therapeutic target for COVID-19. ACE2 exhibits protection from lung injury.

质量

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected ACE2 in 10 ?g of HEK293 cell lysate.

目标描述

~62 kDa observed. Uniport describes 2 isoforms at ~92 kDa (Isoform 1) and ~64 kDa (Isoform 2) due to alternative splicing.

联系

Replaces: MAB5676

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HEK293 cell lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
标签	GST tagged
抗体形式	purified antibody (protein G)
antibody product type	primary antibodies
克隆	4G5.1, monoclonal
分子量	~62 kDa
species reactivity	human
technique(s)	western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_068576
UniProt登记号	Q9BYF1
运输	wet ice
储存温度	2-8℃
Gene Information	human ... ACE2(59272)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable