Anti-3-PGDH Antibody, clone TK-08 13A8

半岛bd体育手机客户端 说明

一般描述

D-3-phosphoglycerate dehydrogenase (EC1.1.1.95; UniProt O43175; also known as 3-PGDH, Epididymis secretory protein Li 113) is encoded by the PHGDH (also known as 3PGDH, HEL-S-113, NLS, NLS1, PGAD, PGDH, PDG, PGD, PGDH3, PHGDHD, SERA) gene (Gene ID 26227) in human. Originally described by Otto Warburg and now commonly known as the Warburg effect refers to the phenomenon that many tumor cells use aerobic glycolysis instead of mitochondrial respiration for energy production to support cell growth and proliferation. Enzymes involved in the complex network of metabolic pathways are therefore being studied as potential targets for cancer treatment. L-serine is an important precursor for macromolecule biosynthesis. 3-PGDH catalyzes the first and rate-limiting step of the serine synthesis pathway from 3-phosphoglycerate to phospho-hydroxypyruvate and is important during tumor development. The PHGDH gene is frequently amplified and the enzyme is overexpressed in 70% of the estrogen receptor-negative breast cancers, 39% of melanoma, and cervical cancers.

免疫原

Recombinant protein corresponding to human 3-PGDH.

应用

Detect PHGDH using this mouse Monoclonal Anti-3-PGDH Antibody, clone TK-08 13A8, Cat. No. MABS1145, validated for use in Western Blotting.
Western Blotting Analysis: 0.5 ?g/mL from a representative lot detected 3-PGDH in 10 ?g HEK293 and Raji cell lysates.

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected 3-PGDH in 10 ?g of HeLa cell lysate.

目标描述

~57 kDa observed

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	TK-08 13A8, monoclonal
species reactivity	human
technique(s)	western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_006614
UniProt登记号	O43175
运输	dry ice
Gene Information	human ... PHGDH(26227)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable