Anti- HSPA8/Hsc70 Antibody, clone N69

半岛bd体育手机客户端 说明

一般描述

Heat shock cognate 71 kDa protein (UniProt P19120; also known as Heat shock 70 kDa protein 8, HSC70, HSC71, HSC73) is encoded by the HSPA8 (also known as HSC70, HSC73) gene (Gene ID 281831) in bovine species. The mammalian HSP70 family consists of the mitochondrial HSP75, the endoplasmic HSP78, the cytoplasmic HSC70 (a.k.a. HSC73 and HSP73) and its inducible counterpart HSP72 (a.k.a. HSP70). The latter two have similar structures and properties, and both of them bind misfolded, newly synthesized polypeptides in an ATP-dependent manner, reflecting their chaperonic activity. All cells constitutively express HSC70. On the other hand, the expression of HSP72 in normal cells is mainly stress inducible, although constitutively high HSP72 expressions are observed in malignant human tumors of various origins. The induced HSP72 expression enables stressed cells to better cope with the elevation of unfolded/denatured proteins and prevent apoptosis under various stress conditions.

特异性

Clone N69 reacts with the constitutively expressed HSPA8/Hsc70 (HSC73), but not the heat schock-inducible HSP72 (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449).

免疫原

Co-purified Hsc73 and Hsp72 proteins from bovine skeletal muscle.

应用

Research Sub Category
Nuclear Receptors
Research Category
Epigenetics & Nuclear Function
Anti- HSPA8/Hsc70 Antibody, clone N69 is an antibody against HSPA8/Hsc70 for use in Western Blotting, Immunocytochemistry, Flow Cytometry, Immunohistochemistry.
Western Blotting Analysis: 2.0 ?g/mL of this antibody detected HSPA8/Hsc70 in 10 ?g rat C6 glioma, human T47D ductal breast epithelial tumor, and human K-562 erythroleukemia cell lysates.
Immunocytochemistry Analysis: 2.0 ?g/mL of a representative lot detected the constitutive HSPA8/Hsc70 expression in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroblastoma cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Flow Cytometry Analysis: 2.0 ?g/mL of a representative lot detected the constitutive HSPA8/Hsc70 expression in C6 rat glioma, B16 mouse melanoma, and K-562 human erythroblastoma cells (Courtesy of Dr. Irina V. Guzhova and Dr. Boris Margulis, Russian Academy of Sciences).
Immunohistochemistry Analysis: A representative lot detected bovine HSPA8/Hsc70 in anterior hypothalamus frozen sections from rats received purified bovine Hsc70 and Hsp72 proteins via intracerebroventricular (i.c.v.) microinjections (Ekimova, I.V., et al. (2010). J. Neurochem. 115(4):1035-1044).
Western Blotting Analysis: A representative lot detected HSPA8/Hsc70 expression in MCF7 cells (Hoyer-Hansen, M., et al. (2005). Cell Death Differ. 12(10):1297-1309).
Western Blotting Analysis: A representative lot detected HSPA8/Hsc70 expression in human breast cancer cell lines (MDA-MB-468, MCF-7, BT-549, and SK-BR-3), non-tumorigenic breast-derived HBL-100 epithelial cells, and WI-38 normal lung fibroblasts (Nylandsted, J., et al. (2000). Proc. Natl. Acad. Sci. U. S. A. 97(14):7871-7876).
Western Blotting Analysis: A representative lot detected HSPA8/Hsc70 (HSC73) purified from bovine skeletal muscle and human myocardium, but not the co-purified HSP72 (Demidov, O.N., et al. (1999). Eur. J. Cardiothorac. Surg. 16(4):444-449).

质量

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 2.0 ?g/mL of this antibody detected HSPA8/Hsc70 in 10 ?g MCF7 cell lysate.

目标描述

~71 kDa observed

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	N69, monoclonal
species reactivity	human, bovine, rat, mouse
technique(s)	flow cytometry: suitable immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_776770
UniProt登记号	P19120
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable