Angiotensin Converting Enzyme-2, His·Tag^?, Human, Recombinant, NSO Cells

半岛bd体育手机客户端 说明

一般描述

Recombinant, human ACE-2 ectodomain (amino acids 1-740) fused at the C-terminus to a His?Tag sequence and expressed in NSO cells. ACE-2 cleaves angiotensins I and II as a carboxypeptidase and plays an important role in regulating cardiovascular and renal functions. Native ACE-2 is an integral membrane zinc metalloprotease whose activity is inhibited by EDTA. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is ~120 kDa under either reducing or non-reducing conditions.
Recombinant, human ACE-2 ectodomain (amino acids 1-740) fused at the C-terminus to a His?Tag sequence and expressed in NSO cells. Native ACE-2 is an integral membrane zinc metalloprotease that can be inhibited by EDTA. ACE-2 cleaves angiotensins I and II. Plays an important role in regulating cardiovascular and renal functions. By SDS-PAGE, the apparent molecular mass of the glycosylated protein is ~120 kDa under either reducing or non-reducing condition.

包装

10 μg in Glass bottle
Please refer to vial label for lot-specific concentration.

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will cleave 1 nmol MCA-YVADAPK(DNP)-OH per min at 25°C, pH 7.5.

重悬

Following initial thaw, aliquot and freeze (-70°C).

其他说明

Tikellis, C., et al. 2003.Hypertension41,390.
Tipnis, S.R., et al. 2000.J. Biol. Chem.275,33238.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
重组	expressed in NSO cells
测定	≥90% (SDS-PAGE)
形式	liquid
specific activity	≥0.8 units/μg protein
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
杂质	≤1.0 EU/μg Endotoxins (EU/μg ACE-2)
运输	wet ice
储存温度	?70℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable