AEC

半岛bd体育手机客户端 说明

一般描述

Precipitable peroxidase substrate for immunoblotting and immunohistochemical staining techniques supplied as a 50X concentrate (0.095 mol/l). To be used with AEC Substrate Buffer, Cat. No. 152226
Precipitable peroxidase substrate for immunoblotting and immunohistochemical staining techniques. Produces a red end product that is soluble in alcohol. When used in immunohistochemistry, aqueous mounting media, and counterstains must be used. Directions included for use in each application.

包装

10 ml in Alu drum

应用

Immunoblotting (see comments)

Immunohistochemistry (see comments)

警告

Toxicity: Toxic (F)

重悬

Discard if a red or brown precipitate is evident.

其他说明

Okano, K., et al. 1992.Rinsho Byori40,93.
Krenacs, T., et al. 1990.Histochem. J.22,530.
Stable at 4°C and 18-26°C. Functionality determined by performing an immunohistologic stain using the final product.



Suggested procedure for use of AEC in immunohistochemistry and immunoblotting:



1. Complete all required incubations with antibodies and HRP-labeled probes, and all necessary wash steps.

2.Prepare 1X AEC Substrate Buffer: Add 9 parts distilled water to 1 part 10X AEC Substrate Buffer, Cat. No. 152226 (0.5 M acetate buffer containing 88 mM H₂O₂). Mix well. The final pH of this solution will be ~5.0.

3. Prepare 1X AEC Substrate Solution: Add 1 part 50X AEC Concentrate to 50 parts 1X AEC Substrate Buffer (prepared in Step 2). Mix well.

4. Following the final wash, completely cover tissue sections with 1X AEC Substrate Solution (prepared in Step 3) and incubate 10-30 min at room temperature. The same conditions apply for immunoblotting. Note: Variables associated with assay conditions will dictate the proper reaction times.

5. After reaction is complete, wash tissue sections or blotting membranes thoroughly in distilled water.

6. If tissue sections require a counterstain, Mayer′s Hematoxylin followed by blueing in alkaline tap water or a 0.5% aqueous methyl green solution is recommended.

7. Mount tissue sections in a water-based mounting medium. Air-dry blots and store protected from light.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C14H14N2
分子量	210.27
MDL编号	MFCD00004964

半岛bd体育手机客户端 性质

质量水平	100
形式	solution
不包含	preservative
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze protect from light
颜色	yellow-brown to reddish
运输	ambient
储存温度	2-8℃
InChI	1S/C14H14N2/c1-2-16-13-6-4-3-5-11(13)12-9-10(15)7-8-14(12)16/h3-9H,2,15H2,1H3
InChI key	OXEUETBFKVCRNP-UHFFFAOYSA-N

安全信息

象形图
警示用语：	Danger
危险声明	H350 - H361d
预防措施声明	P201 - P202 - P280 - P308 + P313 - P405 - P501
危险分类	Carc. 1B - Repr. 2
储存分类代码	6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK	WGK 3