5-DBCO-PEG₄-dUTP, 10mM Aqueous Solution

半岛bd体育手机客户端 说明

应用

5-DBCO-PEG4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR with Taq polymerase. It is enzymatically incorporated into DNA/cDNA as a substitute for its natural counterpart dTTP. The resulting DBCO-functionalized DNA/cDNA can be labeled with azide-labeled molecules using copper-free click chemistry. This enables experiments such as introduction of a biotin group for purification tasks, introduction of a fluorescent group for detection, or crosslinking the DNA to azide-functionalized biomolecules such as proteins.

包装

50 μL in poly bottle

基本信息

经验(实验)分子式	C42H54N5O21P3
分子量	1057.82
PubChem化学物质编号	329769180
NACRES	NA.22

半岛bd体育手机客户端 性质

质量水平	200
测定	>95% (HPLC)
形式	solution
reaction suitability	reaction type: click chemistry
溶解性	Tris buffer: 100 mM (pH 7.5)
运输	wet ice
储存温度	?20℃
SMILES string	O=C1NC(N([C@H]2C[C@@H]([C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O2)O)C=C1/C=C\CNC(CCOCCOCCOCCOCCNC(CCC(N3CC4=CC=CC=C4C#CC5=CC=CC=C53)=O)=O)=O)=O

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2