半岛bd体育手机客户端 说明

一般描述

Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 ?g/ml.
Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H₂O (Cat. No. 508741).

包装

10 mg in Plastic ampoule

生化/生理作用

Product does not compete with ATP.
Cell permeable: no
Primary Target
Binds preferentially to DNA rich in adenine and thymine
Reversible: yes

警告

Toxicity: Standard Handling (A)

重悬

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.

其他说明

Mathieu, C., et al. 1995.Exp. Cell Res.216,13.
Cubria, C., et al. 1994.Comp. Biochem. Physiol. C.105,251.
Hotz, M.A., et al. 1994.Cytometry15,237.
Lee, L.S., and Garnett, H.M. 1993.J. Biochem. Biophys. Methods26,249.
Miller, T., et al. 1993.Biotechniques15,1042.
Gray, J.W., et al. 1982.Science238,323.
Hessling, J.J., et al. 1980.J. Immunol. Methods38,315.
Russels, W.C., et al. 1975.Nature253,461.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

经验(实验)分子式	C16H15N5· 2HCl
分子量	350.25
MDL编号	MFCD00012681

半岛bd体育手机客户端 性质

质量水平	100
测定	≥90% (HPLC)
形式	solid
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze desiccated (hygroscopic) protect from light
颜色	yellow
溶解性	water: 5 mg/mL ethanol: soluble methanol: soluble
荧光	λex~359 nm λem~461 nm
运输	ambient
储存温度	?20℃
InChI	1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key	FPNZBYLXNYPRLR-UHFFFAOYSA-N

安全信息

象形图
警示用语：	Warning
危险声明	H315 - H317 - H335
预防措施声明	P261 - P264 - P271 - P272 - P280 - P302 + P352
危险分类	Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
靶器官	Respiratory system
储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable