Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40

半岛bd体育手机客户端 说明

一般描述

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

特异性

Tyrosine Hydroxylase, phosphoSer40. The antibody recognizes a protein of 60 kDa corresponding to TH phosphorylated at Ser40. Depending on brain region being studied, protein loads and detection methods used some higher molecular weight bands may be detected.
Other species have not been tested.

免疫原

Synthetic peptide from the phosphoSer40 of rat Tyrosine Hydroxylase.
Epitope: phospho-Ser40

应用

Anti-Tyrosine Hydroxylase Antibody, phosphoSer 40 detects level of Tyrosine Hydroxylase & has been published & validated for use in IC, IH & WB.
Research Category
Neuroscience
Immunohistochemistry:
A 1:1,000 dilution of a previous lot was used in IH.

Immunocytochemistry:
A 1:1,000 dilution of a previous lot was used in IC.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Neurotransmitters & Receptors

质量

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected TYROSINE HYDROX PSER40 on 10 μg of PC12 lysates.

目标描述

60 kDa

外形

ImmunoAffinity Purified
Purified rabbit polyclonal liquid in buffer containing 10mM Hepes, pH 7.5, 150mM NaCl, 100 ?g/mL BSA, and 50% glycerol.

储存及稳定性

Stable for 6 months at -20°C in undiluted from date of receipt.

分析说明

Control
POSITIVE CONTROL: brain (sympathetic nerve terminals) and adrenal glands.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	avian, mouse, rat
species reactivity (predicted by homology)	nonhuman primates (100% sequence homology), human (100% sequence homology), quail (100% sequence homology), porcine (100% sequence homology)
manufacturer/tradename	Chemicon?
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable western blot: suitable
NCBI登记号	NM_000360.3
UniProt登记号	P07101
运输	dry ice

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2