Anti-phospho-p70 S6 Kinase (Thr389) Antibody, clone 10G7.1

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一般描述

The p70 S6 kinase is a ubiquitous cytoplasmic protein that is activated in response to cytokines, and growth factors including the epidermal growth factor and fibroblast growth factor. It lies downstream of the mTOR/PI3K pathway and is phosphorylated on multiple residues: Thr389, Thr229, Thr421, Ser371, Ser404, Ser411, Ser418, and Ser424. Thr389, Ser371, and Ser404 are located in the linker domain; whereas Thr421, Ser411, Ser418, Ser424 are located in the autoinhibitory domain, and Thr229 is found in the catalytic domain. These sites may be phosphorylated in a hierarchical sequence leading to the activation of the p70 S6 kinase. The p70 S6 kinase phosphorylates the S6 protein on the 40S ribosomal protein (rpS6), and plays a role in multiple processes including regulation of mRNA molecules with 5’-terminal oligopyrimidine tracts, protein synthesis, cell growth, proliferation, and cell survival. The activity of this kinase may be important in diabetes, cancer, and aging.

特异性

This antibody recognizes p70 S6 Kinase phosphorylated at (Thr389). This antibody also recognizes isoforms Alpha I and Alpha II.

免疫原

Epitope: Phosphorylated (Thr389)
KLH-conjugated linear peptide corresponding to mouse p70 S6 Kinase phosphorylated at (Thr389).

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
This Anti-phospho-p70 S6 Kinase (Thr389) Antibody, clone 10G7.1 is validated for use in Western Blotting, ICC for the detection of phospho-p70 S6 Kinase (Thr389).
Research Category
Signaling
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected p70 S6 Kinase in A431 cells.

质量

Evaluated by Western Blot in untreated and serum treated NIH/3T3 cell lysates.

Western Blot Analysis: 1 ?g/mL of this antibody detected p70 S6 Kinase on 10 ?g of untreated and serum treated NIH/3T3 cell lysates.

目标描述

~70/85 kDa observed. This protein recognizes isoforms Alpha I and Alpha II. Uncharacterized bands appear at ~18 and 200 kDa in some lysates depending on treatment conditions.

联系

Replaces: 04-392

外形

Format: Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Untreated and serum treated NIH/3T3 cell lysates

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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