Anti-Glial fibrillary acidic protein δ Antibody

半岛bd体育手机客户端 说明

一般描述

Human Glial Fibrillary Acidic Protein delta (GFAP-delta) is a GFAP protein isoform that is encoded by an alternative splice variant of the GFAP-gene. As a result, GFAP-delta protein differs from the predominant splice form, GFAP-alpha, by its C-terminal protein sequence. GFAP-delta protein is not expressed by all GFAP expressing astrocytes but specifically by a subpopulation located in the subpial zone of the cerebral cortex, the subgranular zone of the hippocampus and, most intensely, by a ribbon of astrocytes following the ependymal layer of the cerebral ventricles. Therefore, at least in the sub ventricular zone (SVZ), GFAP-delta specifically marks the population of astrocytes that contain the neural stem cells in the adult human brain. Interestingly, the SVZ astrocytes actively splice GFAP-delta transcripts, in contrast to astrocytes adjacent to this layer. Data shows that GFAP-delta protein, unlike GFAP-alpha, is not upregulated in astrogliosis. Data indicates a different functional role for GFAP-delta in astrocyte physiology. Transfection studies showed that GFAP-delta protein expression has a negative effect on GFAP filament formation, and therefore could be important for modulating intermediate filament cytoskeletal properties, possibly facilitating astrocyte motility. Further studies on GFAP-delta and the cells that express it are important for gaining insights into its function during differentiation, migration and during health and disease (Roelofs, RF, et al., Glia (2005) 52:289-300.).

特异性

Glial Fibrillary Acidic Protein-delta (GFAP-delta). The antibody does not recognize GFAP-alpha protein.

免疫原

Synthetic peptide from human GFAP-delta.

应用

Research Sub Category
Neuronal & Glial Markers
Research Category
Neuroscience
Western blot: 1:500 with overnight incubation. The antibody recognizes the ~60 kDa GFAP-delta protein.

Immunohistochemistry: 1:500 incubated for 36-48 hours at 2-8°C on paraffin embedded tissue sections. It is suggested that the tissue be treated with microwave antigen retrieval prior to staining.

Optimal working dilutions must be determined by the end user.
Anti-Glial fibrillary acidic protein δ Antibody is an antibody against Glial fibrillary acidic protein δ for use in WB, IH(P).

联系

Replaces: 04-1031; 04-1062

外形

Rabbit serum. Liquid.

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	serum
antibody product type	primary antibodies
克隆	polyclonal
species reactivity	human
should not react with	mouse, rat
manufacturer/tradename	Chemicon?
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
NCBI登记号	NM_002055.2
UniProt登记号	P14136
运输	dry ice
Gene Information	human ... GFAP(2670)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable