Anti-CSF-1R/CD115 Antibody clone FER216

半岛bd体育手机客户端 说明

一般描述

Macrophage colony-stimulating factor 1 receptor (EC 2.7.10.1; UniProt P07333; also known as CD115, CSF-1 receptor, CSF-1-R, CSF-1R, M-CSF-R, Proto-oncogene c-Fms) is encoded by the CSF1R (also known as CSFR, FMS, HDLS) gene (Gene ID 1436) in human. CSF1R is a CSF1/PDGF family receptor tyrosine kinase that mediates macrophage colony stimulating factor 1 (M-CSF1) and IL-34 signaling by acting as the receptor for these two cytokines. CSF1R plays a key role in the differentiation, recruitment and activation of tissue macrophages. Tumor-associated macrophages (TAMs) have been associated with survival in classic Hodgkin lymphoma (cHL) and other lymphoma types. Hyperphosphorylated CSF1R is found in cHL tissue and highlights the importance of CSF1/CSF1R signaling in the recruitment of TAMs. CSF1R is initially produced with a signal peptide sequence (a.a. 1-19), the removal of which yields the mature type I single transmembrane protein with an extracellular region (a.a. 20-517), a transmembrane segment (a.a. a.a. 518-538), and a cytoplasmic region (a.a. 539-972) that contains the kinase domain (a.a. 582-910).

特异性

Clone FER216 reacts with the extracellular domain of human CSFR1, but not the extracellular domain of human FGFR1. Clone FER216 detected the ~100 kDa human CSFR1 in nomral tonsil and Hodgkin lymphoma (cHL) tissue extracts, as well as an additional ~150 kDa phosphorylated CSFR1 band in cHL tissue extracts (Martín-Moreno, A.M., et al. (2015). PLoS One. 2015 Jun 12;10(6):e0125203).

免疫原

Human IgG1 Fc-tagged recombinant human CSF-1R/CD115 extracellular domain.
Epitope: Extracellular domain.

应用

Research Sub Category
Cytokines & Cytokine Receptors
Anti-CSF-1R/CD115 Antibody clone FER216 is an antibody against CSF-1R for use in Western Blotting.
Western Blotting Analysis: 1.0 ?g/mL from a representative lot detected CSF-1R/CD115 in 10 ?g of human tonsil tissue lysate.
Research Category
Signaling

质量

Evaluated by Western Blotting in human colon tissue lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected CSF-1R/CD115 in 10 ?g of human colon tissue lysate.

目标描述

~107 kDa observed. Uncharacterized band(s) may appear in some lysates.

外形

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	FER216, monoclonal
species reactivity	human
technique(s)	western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_005202
UniProt登记号	P07333
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable