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位置: 首页> 品牌> Sigma-Aldrich> Anti-Interleukin-8 Antibody

Anti-Interleukin-8 Antibody

品牌
Sigma-Aldrich
货号
AB1840
规格纯度
serum, Chemicon?
价格
4513.21 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-Interleukin-8 Antibody
IL-8
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

特异性

Reacts with ovine IL-8. No reactivity detected to recombinant ovine IL-1beta, IL-6, MCP or TNFalpha.

免疫原

Recombinant ovine IL-8

应用

Detecting antibody in ELISA, using monoclonal anti-ovine IL-8 as capture antibody. Recommended dilution factor 1:500. See suggested protocol.

Optimal working dilutions must be determined by the end user.

Suggested ELISA Protocol For Ovine IL-8

1. Coat high capacity microtiter wells (e.g. Nunc maxisorp) with monoclonal anti-IL-8 antibody (Chemicon Catalog Number MAB1044) at a concentration of 5 μg/mL in 0.1 M carbonate buffer pH 9.6 overnight at 4?C.

2. Wash wells 3 x with PBS + 0.05% Tween 20 (PBST).

3. Add samples to wells. Incubate at room temperature in a humidified box for 1 hour. Wash 3 x with PBST.

4. Dilute rabbit anti-IL-8 (Chemicon Catalog Number AB1840) 1:500 in PBST and add 100 μL to wells. Incubate at room temperature for 30 minutes. Wash 3 x with PBST.

5. Dilute anti-rabbit IgG-HRP to appropriate level in PBST and add 100 μL to wells. Incubate 30 minutes at room temperature. Wash 3 x with PBST.

6. Add 100 μL of TMB chromagen to each well. Incubate for 5-10 minutes and stop reaction with 50 μL of 2M sulfuric acid.

Notes:

The anti-rabbit IgG HRP must be adsorbed against mouse immunoglobulin, and should be checked for non-reactivity with coated plates.

Non-specific binding may be reduced by addition of protein such as skim milk powder to the diluents.

Assay sensitivity may be altered by adjusting incubation times and reagent concentrations.
Anti-Interleukin-8 Antibody detects level of Interleukin-8 & has been published & validated for use in ELISA.
Research Sub Category
Cytokines & Cytokine Receptors
Research Category
Inflammation & Immunology

外形

Rabbit serum. Liquid containing 0.1% sodium azide.

储存及稳定性

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 serum
antibody product type primary antibodies
克隆 polyclonal
species reactivity sheep
manufacturer/tradename Chemicon?
technique(s) ELISA: suitable
NCBI登记号 NM_000584.2
UniProt登记号 P10145
运输 dry ice

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 1

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