Anti-Insulin Receptor Antibody, beta subunit, clone CT-3

半岛bd体育手机客户端 说明

一般描述

The insulin receptor is a tyrosine kinase receptor that when bound to insulin, initiates multiple signal transduction pathways, including JNK, PI 3-kinase, Akt and PKC. Pharmacological intervention of these Insulin R-dependent pathways is of great interest for the treatment of insulin resistance, obesity and diabetes. The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α-chain and a transmembrane and intracellular β-chain, tethered together by disulfide bonds. The β-chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. The IR signals primarily by phosphorylating the Insulin Receptor Substrate (IRS) family of proteins, which creates docking sites for SH2-domain containing proteins. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin.

特异性

This antibody recognizes the C-terminus of human Insulin Receptor, beta subunit.

免疫原

Recombinant protein corresponding to the C-terminus of human Insulin Receptor, beta subunit.
Epitope: C-terminus

应用

Research Category
Metabolism
Detect Insulin Receptor using this Anti-Insulin Receptor Antibody, beta subunit, clone CT-3 validated for use in WB, IH.
Research Sub Category
Insulin/Energy Signaling

质量

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 0.5 ?g/ml of this antibody detected Insulin receptor beta subunit on 10 ?g of mouse brain tissue lysate.

目标描述

95 kDa

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	CT-3, monoclonal
species reactivity	rat (demonstrated by independent lab), human, mouse
technique(s)	immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_000199.2
UniProt登记号	P06213
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable