W6/1

半岛bd体育手机客户端 说明

细胞系描述

The W6/1 hybridoma cell line secretes monoclonal IgM antibodies reactive with a polymorphic determinant of human erythrocytes which correlated with blood group A.

免疫原

Human tonsil membrane

应用

Human erythrocyte marker.

培养基

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

传代培养常规

Seed at 5 x 10⁴cells/ml. Subculture when cells reach 5 x 10⁵-1 x 10⁶cells/ml (usually after 3-4 days), 5% CO₂, 37℃. Start in 20% FBS when recovering from frozen.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

半岛bd体育手机客户端 性质

生物来源	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
骨髓瘤	P3-NS1/1-Ag4.1
反应性	Human ′A′ erythrocytes
technique(s)	cell culture | mammalian: suitable
同位素/亚型	IgM
运输	dry ice
储存温度	?196℃