OX-82

半岛bd体育手机客户端 说明

细胞系描述

The hybridoma cell line OX-82 secretes monoclonal IgG1 antibodies reactive with an uncharacterised neutrophil marker.

免疫原

Rat bone marrow cells

应用

Detection of neutrophils.

培养基

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

传代培养常规

Seed at 5 x 10⁴cells/ml (5 x 10⁵cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵- 1 x 10⁶cells/ml (usually after 3-4 days), 5% CO₂, 37℃. 20% FBS can be used at resuscitation.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

半岛bd体育手机客户端 性质

生物来源	Mouse x Mouse Hybridoma
ImmunoDon	BALB/c mouse spleen
骨髓瘤	NS1
反应性	Rat neutrophils, erythrocyte stem cells, 50% bone marrow
technique(s)	cell culture | mammalian: suitable
同位素/亚型	IgG1
运输	dry ice
储存温度	?196℃