Thioredoxin, Human, Recombinant,E. coli

半岛bd体育手机客户端 说明

一般描述

Recombinant, human thioredoxin expressed inE. coli. A ubiquitous protein with two redox-active half-cysteine residues that function as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman′s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T-cells and human PBL blasts. Occurs as a single polypeptide chain.
Recombinant, human thioredoxin expressed inE. coli. A ubiquitous protein with two redox-active half-cysteine residues. Functions as a general disulfide reductant. Works efficiently on model compounds (e.g. Ellman’s reagent, Cat. No. 322123) and protein disulfides. Plays an essential role in cellular protection against oxidative stress and cell death. Inhibits apoptosis in Jurkat T cells and human PBL.

包装

100 μg in Plastic ampoule

警告

Toxicity: Standard Handling (A)

单位定义

One unit (insulin reduction unit) is calculated by measuring the change in absorbance at 650 nm with 330 ?M DTT as the reductant at 25°C, pH 6.5.

重悬

Reconstitute in physiological buffer containing 3 mM EDTA and 1-5 mM DTT.
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.

其他说明

Noda, N., et al. 1997.Nippon Rinsho55,461.
Wollman, E.E., et al. 1997.Biochem. Biophys. Res. Commun.230,602.
Masutani, H., et al. 1996.Immunol. Lett.54,67.
Saito, I., et al. 1996.Arthritis Rheumatism,39,773.
Saito, N., et al. 1995.J. Immunol.154,3194.
Buchanon, B.B., et al. 1994.Arch. Biochem. Biophys.314,257.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

基本信息

MDL编号

MFCD00132423

半岛bd体育手机客户端 性质

质量水平	100
重组	expressed inE. coli
测定	≥90% (SDS-PAGE)
形式	lyophilized solid
specific activity	≥1.7 units/mg protein
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
运输	ambient
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable