半岛bd体育手机客户端 说明
一般描述
Contains a reactive functional group (allylic bromide) that is capable of reacting with a wide variety of nucleophiles to form stable covalent adducts. Reacts extremely rapidly with thiol groups under physiological conditions to yield highly fluorescent products. A labeling reaction is typically carried out at room temperature for 2 to 60 min, pH 7.4, using an equivalent amount (or a small excess) of reagent relative to the amount of thiol. Material is chemically and photolytically stable and shows an absorption maxima at 370 to 385 nm and emits light at 477 to 484 nm (turquoise). Readily penetrates membranes. Useful as an alternative tool in labeling methodology, complementing the use of such well known fluorescent labeling reagents as fluorescein and rhodamine isothiocyanates (whose reactions are slower and yield products that fade relatively fast during irradiation) and fluorescamine (which reacts primarily with amine groups, but whose products also fade relatively rapidly).
Has been used to labelE. colithioredoxin. Weakly fluorescent but reacts with thiols to yield highly fluorescent stable thioethers.
包装
25, 100, 250 mg in Plastic ampoule
警告
Toxicity: Standard Handling (A)
制备说明
Recommended stock solution is 13 mg/ml (50 mM) in acetonitrile.
重悬
Following reconstitution, refrigerate (4°C). Stock solutions are stable for up to 3 months at 4°C. Aqueous stock solutions have a half-life of ~4 h at room temperature (20°C).
其他说明
Mansoor, M.A., et al. 1992.Anal. Biochem.200,218.
Zangerle, L., et al. 1992.J. Neurochem.59,181.
Agarwal, R. and Burke, M. 1991.Arch. Biochem. Biophys.290,1.
Martin, R.L., et al. 1989.J. Biol. Chem.264,11768.
Bonet, A., et al. 1988.Biochem. Biophys. Res. Commun.152,1.
Chinn, P.C. 1985.Fed. Proc.44,1620.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
THIOLYTE is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| MDL编号 | MFCD00036951 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥95% (HPLC) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze protect from light |
| 颜色 | yellow |
| 溶解性 | acetonitrile: 13 mg/mL methanol: 25 mg/mL |
| 运输 | ambient |
| 储存温度 | 10-30℃ |
| InChI | 1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3 |
| InChI key | AHEWZZJEDQVLOP-UHFFFAOYSA-N |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
