SILu^TMMAb Mouse Stable-Isotope Labeled Monoclonal Antibody

半岛bd体育手机客户端 说明

一般描述

SILu^?MAb Mouse is a recombinant, stable isotope-labeled, mouse monoclonal antibody which incorporates [¹³C₆,¹⁵N₄]-Arginine and [¹³C₆,¹⁵N₂]-Lysine. Expressed in CHO cells, SILu^?MAb Mouse is designed to be used as a universal internal standard for bioanalysis of monoclonal murine antibodies in animal pharmacokinetics (PK) studies.

SILu^?MAb Mouse is an IgG1 antibody with a kappa light chain. Because of overlap with common sequences in the Fc region with candidate murine antibodies, SILu^?MAb Mouse provides universal utility, and thus, eliminates the need to produce candidate-specific internal standards.

外形

Each vial of SILu^?MAb Mouse contains the labeled antibody lyophilized from a solution of phosphate buffered saline. Vial content was determined by measuring A₂₈₀and using an extinction coefficient (E_0.1%) of 1.4.

制备说明

Produced utilizing enriched media containing stable isotope labeled amino acids such as [¹³C₆,¹⁵N₄]-Arginine and [¹³C₆,¹⁵N₂]-Lysine. Expressed in CHO cells, SILuMAb Mouse is designed to be used as a universal internal standard for bioanalysis of monoclonal murine antibodies in animal pharmacokinetics (PK) studies

重悬

SILu^?MAb Mouse recovery is maximized when 0.1% formic acid is used for reconstitution of the lyophilized product.
Reconstitution with other solvents may reduce recovery. Do not freeze after reconstitution.

• Briefly centrifuge the vial at ~10,000 × g to collect the product at the bottom of the vial.
• Add 500 μL of ultrapure water containing 0.1% formic acid to the vial.
• Mix the contents by gently inverting the vial a minimum of 5 times.
• Allow the vial to stand at room temperature for at least 15 minutes and repeat mixing by inversion.

法律信息

SILu is a trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
重组	expressed in CHO cells
antibody product type	primary antibodies
测定	≥90% (SDS-PAGE)
包装	vial of 100 μg
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable