PhosphoDetect Anti-VASP (pSer¹⁵⁷) Mouse mAb (5C6)

半岛bd体育手机客户端 说明

一般描述

Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.
This PhosphoDetect Anti-VASP (pSer???) Mouse mAb (5C6) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer???).
Mouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the the ~50 kDa VASP protein phosphorylated at Ser¹⁵⁷.

免疫原

Human
a synthetic phosphopeptide corresponding to amino acids surrounding the Ser??? phosphorylation site of human VASP

包装

100 μg in Plastic ampoule

应用

ELISA (0.05 ?g/ml)

Immunoblotting (1 ?g/ml, colorimetric; 0.1 ?g/ml, chemiluminescence)

Immunocytochemistry (1-10 ?g/ml)

Immunoprecipitation (1-10 ?g/10? vanadate-treated A431 cells)

警告

Toxicity: Standard Handling (A)

外形

Lyophilized from PBS, PEG, sucrose.

重悬

Reconstitute in 1 ml sterile, dH?O, 15 min, room temperature. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.

分析说明

Positive Control
Vanadate-treated A431 cells

其他说明

Does not cross-react with non-phosphorylated VASP or with unrelated phosphorylation sites. For immunocytochemistry, fixation protocol may include up to 0.5% formaldehyde. Variables associated with assay conditions will dictate the proper working dilution.
Bear, J.E., et al. 2001.Curr. Opin. Cell. Biol.13,158.
Lawrence, D.W., and Pryzwansky, K.B. 2001.J. Immunol.166,5550.
Burkhardt, M., et al. 2000.J. Biol. Chem.275,33536.
Harbeck, B., et al. 2000.J. Biol. Chem.275,40.
Smolenski, A., et al. 2000.J. Biol. Chem.275,25723.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	5C6, monoclonal
形式	lyophilized
包含	≤0.1% sodium azide as preservative
species reactivity	human, mouse
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze
同位素/亚型	IgG1
运输	ambient
储存温度	?20℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable