半岛bd体育手机客户端 说明
一般描述
Purified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.
Peroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
This PhosphoDetect
免疫原
KLH conjugated to phosphotyrosine, glycine, and alanine
包装
100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
应用
ELISA (1:2000)
Immunoblotting (1:500-1:2000)
Immunohistochemistry (1:500)
警告
Toxicity: Standard Handling (A)
外形
In PBS, 50% glycerol, 1% BSA, pH 7.4.
其他说明
Glenney, J.R., et al. 1988.J. Immunol. Methods109,277.
Specific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins.Do not use milk as a blocking agent with this antibody.Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with <150 mM NaCl and divalent cations not much >1 mM because this antibody′s binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | PY20, monoclonal |
| 形式 | liquid |
| 包含 | 0.1% Proclin as preservative |
| species reactivity (predicted by homology) | all |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze |
| 同位素/亚型 | IgG2b |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
