PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20)

半岛bd体育手机客户端 说明

一般描述

Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
Purified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
This PhosphoDetect Anti-Phosphotyrosine Mouse mAb (PY20) is validated for use in ELISA, Immunoblotting, Frozen Sections, ICC, IP for the detection of Phosphotyrosine.

免疫原

KLH conjugated to phosphotyrosine, glycine, and alanine

包装

100 μg in Plastic ampoule
Please refer to vial label for lot-specific concentration.

应用

ELISA (see comments)

Immunoblotting (1:500-1:1000, see application references)

Frozen Sections (see application references)

Immunocytochemistry (see application references)

Immunoprecipitation (see comments)

警告

Toxicity: Standard Handling (A)

外形

In 10 mM sodium phosphate, pH 7.4.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Positive Control
Any cell lysate or tissue extract

其他说明

Glenney, J.R., et al. 1988.J. Immunol. Methods109,277.
Binding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins.Do not use milk as a blocking agent with this antibody.Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	PY20, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity (predicted by homology)	all
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
同位素/亚型	IgG2b
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable