PEG4-SPDP (PEGylated, long-chain SPDP crosslinker)

半岛bd体育手机客户端 说明

一般描述

SPDP-type reagents have an amine-reactive N-hydroxysuccinimide (NHS) ester at one end and a suflhydryl-reactive 2-pyridyldithiol group at the opposite end. PEG4-SPDP has a 4-unit polyethylene glycol spacer arm, which confers greater solubility to the crosslinker and linked proteins compared to crosslinkers having only hydrocarbon spacers. Pyridyldithiol reagents produce disulfide-containing linkages that can be cleaved with reducing agents such as dithiothreitol (DTT). Crosslinking experiments with SPDP reagents are not limited to those involving proteins. Any of a variety of molecules with primary amines and sulfhydryl groups can be modified or crosslinked using an SPDP reagent.

特点和优势

• Reactive groups: NHS ester and pyrirdyldithiol
• Efficient amine conjugation—NHS ester provides effective conjugation to lysines, N-termini of peptides and other primary amines
• Reversible sulfhydryl conjugation—pyridyldithiol group enables disulfide linkage with reduced cysteines and other sulfhydryl groups, providing a stable but cleavable bond
• Polyethylene glycol—PEG groups are flexible, non-immunogenic, hydrophilic, and often enhance the solubility of attached molecules
• Spacer arm—connects primary amines and sulfhydryl targets at distances up to 25.7 angstroms; compare to PEG12-SPDP (54.1 angstroms)

注意

This product is sensitive to moisture. The vial is packaged in a resealable bag with a desiccant to reduce exposure to moisture. After cold storage, equilibrate the vial to room temperature before opening to reduce condensation inside the vial. Make fresh solutions. Storage of stock solutions is not recommended. After use, return the vial to the resealable bag. Close the bag and store the product at the recommended temperature.

基本信息

经验(实验)分子式	C23H33N3O9S2
分子量	559.65

半岛bd体育手机客户端 性质

质量水平	100
分子量	559.65
reaction suitability	reagent type: cross-linking reagent
储存条件	desiccated
溶解性	water: soluble
运输	ambient
储存温度	?20℃
SMILES string	O=C(CCSSC1=CC=CC=N1)NCCOCCOCCOCCOCCC(ON2C(CCC2=O)=O)=O

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable