MystiCq^?microRNA qPCR Assay Primer

半岛bd体育手机客户端 说明

一般描述

The MystiCq^?microRNA qPCR Assay Primer is an integral part of the MystiCq microRNA qPCR Assay System. It has been designed to target specific microRNAs with the MystiCq Universal PCR primer and the MystiCq^?microRNA SYBR^?Green qPCR ReadyMix^?on cDNA templates generated by the MystiCq microRNA cDNA Synthesis Mix kit.

生化/生理作用

The microRNA mmu-miR-21-3p has an anti-apoptotic function in bone marrow mesenchymal stem cells. It has been shown that this microRNA is highly modulated in mice with diabetic nephropathy. Inhibition of miR-21 could be a target for fibrosis development in cardiac allografts.

特点和优势

• Proprietary design features of assay primers allow for specificity towards mature microRNAs vs. precursor-microRNAs
• Extensive design and testing of oligo-dT adapter primer to incorporate complementary Universal PCR Primer sequence
• No self-complementarity or primer dimer artifacts with the Universal PCR Primer
• Optimized primer Tm designed to match the Universal PCR Primer
• Universal cycling conditions ensures robust amplification for all assays in profiling experiments
• Optimized PCR product Tm (75-78° C)

其他说明

Formerly mmu-miR-21*. Compatible with rno-miR-21*.

法律信息

MystiCq is a registered trademark of Qiagen Beverly Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC
SYBR is a registered trademark of Life Technologies

基本信息

miRNA ID	mmu-miR-21-3p Sanger Database
NACRES	NA.55

半岛bd体育手机客户端 性质

形式	liquid
浓度	10 mg/mL
成熟序列	CAACAGCAGUCGAUGGGCUGUC
mp	~76 ℃
Sanger mature/minor 登记号	MIMAT0004628
运输	wet ice
储存温度	?20℃
Gene Information	mouse ... MIR21A(387140)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable