半岛bd体育手机客户端 说明
一般描述
A specific fluorogenic resonance energy transfer (FRET) peptide substrate with an MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity [kcat/Km= 619,000 M-1s-1for MMP-2, 206,000 M-1s-1for MMP-9, 40,000 M-1s-1for MMP-3, and 21,000 M-1s-1for MMP-1; at 37°C, pH 7.6 ]. Exhibits a high degree of sensitivity that is not affected by optical disturbances in biological media. Also useful for MMP activity measurements in synovial fluid and culture medium.
A fluorescence energy transfer (FRET) peptide substrate with MMP cleavable Gly-Leu bond and EDANS/Dabcyl as fluorophore/quencher combination. Useful for the detection of MMP activity (kcat/Km= 619,000 M-1s-1for MMP-2, 206,000 M-1s-1for MMP-9, 40,000 M-1s-1for MMP-3, 21,000 M-1s-1for MMP-1; pH = 7.6 at 37°C). Exhibits a high degree of sensitivity, which is not affected by optical disturbances in biological media. Excitation max.: ~340 nm; emission max.: ~485 nm.
包装
500 μg in Plastic ampoule
Packaged under inert gas
生化/生理作用
Primary Target
Detection of MMP activity
Product does not compete with ATP.
Reversible: no
Cell permeable: no
警告
Toxicity: Standard Handling (A)
序列
DABCYL-GABA-Pro-Gln-Gly-Leu-Glu(EDANS)-Ala-Lys-NH?
外形
Supplied as a trifluoroacetate salt.
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
其他说明
Beekman, B., et al. 1996.FEBS Lett390,221.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C63H88N16O14S |
| 分子量 | 1325.54 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 测定 | ≥97% (HPLC) |
| 形式 | lyophilized solid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze protect from light |
| 颜色 | red |
| 溶解性 | DMSO: soluble Tris-buffered saline, pH 7.6: soluble |
| 荧光 | λex~340 nm λem~485 nm |
| 运输 | ambient |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
