Lys-c (lysyl-endopeptidase), Active

半岛bd体育手机客户端 说明

一般描述

Recombinant tag-freeAchromobacter lyticusLys-c (lysyl-endopeptidase) (206-473aa) was expressed inE.colicells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacteriumAchromobacter lyticusby Msakiet al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

特异性

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 ℃.

应用

The enzyme functions optimally between 30-37 ℃ and suffers from degradation when subjected to temperatures above 50 ℃. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 ℃.

包装

1mg/ml in Plastic

制备说明

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 ℃.

重悬

Reconstitute in sterile water.

储存及稳定性

Store product at -20℃. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles once the protein has been resolubilized in sterile water.

其他说明

For R&D only.

基本信息

酶学委员会(EC)编号

3.4.21.50 ( BRENDA | IUBMB )

半岛bd体育手机客户端 性质

质量水平	200
重组	expressed inE. coli(206-473aa)
形式	lyophilized powder
浓度	1 mg/mL
最佳pH	8.5
pH范围	9.0 - 9.5 (catalytic)
UniProt登记号	P15636
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H315 - H317 - H319 - H334
预防措施声明	P261 - P264 - P272 - P280 - P302 + P352 - P305 + P351 + P338
危险分类	Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码	11 - Combustible Solids
WGK	WGK 3