Lys-c (lysyl-endopeptidase), Active

半岛bd体育手机客户端 说明

一般描述

Recombinant tag-freeAchromobacter lyticusLys-c (lysyl-endopeptidase) (206-473aa) was expressed inE.colicells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacteriumAchromobacter lyticusby Msakiet al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

特异性

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 ℃.

应用

The enzyme functions optimally between 30-37 ℃ and suffers from degradation when subjected to temperatures above 50 ℃. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 ℃.

包装

1mg/ml in Plastic

制备说明

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 ℃.

储存及稳定性

Store product at -20℃ for up to one year. Avoid freeze/thaw cycles.

其他说明

For R&D only.

基本信息

酶学委员会(EC)编号

3.4.21.50 ( BRENDA | IUBMB )

半岛bd体育手机客户端 性质

质量水平	200
生物来源	Achromobacter lyticus
重组	expressed inE. coli(206-473aa)
形式	buffered aqueous glycerol solution
浓度	1 mg/mL
最佳pH	8.5
pH范围	9.0 - 9.5 (catalytic)
UniProt登记号	P15636
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable