半岛bd体育手机客户端 说明

一般描述

LDH (lactic dehydrogenase), a glycolytic enzyme, particularly present in skeletal muscle, heart, liver, kidneys, brain, lungs and red blood cells. It has five isoenzyme forms. LDH possess a tetrameric structure.

应用

Lactic Dehydrogenase, recombinant fromE. colihas been used:

• in lactate dehydrogenase (LDH) and malate dehydrogenase 1 (MDH1)assays and cross-linking assays
• to prepare assay buffer to measure pyruvate kinase (PYK) by coupled assay
• inin vitroDltC D -alanylation assay

生化/生理作用

Conversion of L -lactate into L -pyruvate is crucial in hypoxic and anaerobic conditions, especially when synthesis of adenosine triphosphate (ATP) by oxidative phosphorylation is interrupted.
L -乳酸脱氢酶催化 L -乳酸转化为 L -丙酮酸，同时还原 NAD⁺为 NADH 和 H⁺。

单位定义

One unit corresponds to the amount of enzyme which reduces 1 μmol pyruvate per minute at pH 7.4 and 25℃ (NADH as cofactor)

基本信息

半岛bd体育手机客户端 性质

安全信息